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EnzyChrom™ Pyruvate Kinase Assay Kit
Application
- For quantitative determination of pyruvate kinase (PK) activity and evaluation of drug effects on PK activity.
Key Features
- Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 50 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays run at 25°C for 30 min.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD570nm, FL530/590nm
Samples
- Plasma, serum, cell, and tissue, etc
Species
- All
Procedure
- 40 min
Size
- 100 tests
Detection Limit
- 0.01 U/L
Shelf Life
- 6 months
More Details
Pyruvate Kinase (PK) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, a genetic disease, is caused by a lack of pyruvate kinase and slows down the process of glycolysis. Pyruvate kinase is also involved in gluconeogenesis, a biochemical pathway in which the liver generates glucose from pyruvate and other substrates. BioAssay Systems’ Pyruvate Kinase Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity. In this assay, PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/590nm is directly proportional to the pyruvate generated by the Pyruvate Kinase in the sample.No frequently asked questions for this new product. Please check back later.
For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$519.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
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| Name | SKU | Price | Buy |
|---|---|---|---|
| EnzyChrom™ Pyruvate Assay Kit | EPYR-100 | $419.00 |
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