Phosphatase Inhibitor

ProtocolSodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems’ vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays.

Key Features

• Ready-to-use phosphatase inhibitor.
• Fully activated for maximum inhibition.

Size

• 1 mL 100 mM

Shelf Life

• 3 years

More Details

Sodium Orthovanadate (CAS Number 13721-39-6, Na₃VO₄) is a general inhibitor for protein phosphotyrosyl phosphatases (PTPs). Inhibition of vanadate is competitive and can be reversed by addition of EDTA or by dilution. Vanadate is commonly used to preserve the protein tyrosyl phosphorylation state in cells and lysates. BioAssay Systems Vanadate reagent is activated for maximum inhibition. Suggested Working Concentration: 1-10 mM

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

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