EnzyChrom™ Phenylalanine Assay Kit

Protocol SDS

Application

• For quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. Linear detection range of 2 – 300 µM L-phenylalanine.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/595nm

Samples

• Serum, urine etc

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 2 µM

Shelf Life

• 6 months

More Details

L-Phenylalanine is one of the twenty common amino acids and an important precursor for several key signal molecules such as dopamine, norepinephrine, epinephrine, and the skin pigment melanin. It is found naturally in the breast milk of mammals and used as a nutritional supplement in food and drink products. The genetic disorder phenylketonuria is the inability to metabolize phenylalanine. Individuals with this disorder are known as “phenylketonurics”. Individuals who cannot metabolize phenylalanine must monitor their intake of protein to control the buildup of phenylalanine. BioAssay Systems’ L-Phenylalanine Assay Kit provides a convenient fluorimetric means to measure L-phenylalanine in biological samples. In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. The resulting fluorescence intensity (λex/em = 530/585 nm) is linear to the L-phenylalanine concentration in the sample.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

DeRosa, F., Heartlein, M., & Dias, A. (2016). MRNA therapy for phenylketonuria. U.S. Patent No. 9,522,176. Assay: Phenylalanine in human serum.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.