The High, Medium, and Low Controls are 10 mM stock solutions of Methyl Paraben, and Propyl Paraben, and Theophylline, respectively, in DMSO.
Pipetting devices, DMSO, PBS, UV Plates (Cat # P96UV), and an absorbance plate reader capable of absorbance spectra.
The donor plate membrane is a Hydrophobic PVDF membrane with a pore size of 0.45 µm.
We recommend against using higher concentrations of DMSO in the PAMPA assay. The spectrophotometric background in the Acceptor solution will increase with higher DMSO concentrations, and the higher concentration of DMSO may alter the membrane and affect the result.
If your compound(s) is/are insoluble in PBS with 5% DMSO, clarify the solution by centrifugation at 13,000xg for 10 minutes then use the supernatant for the Donor solution. For the Equilibrium Standard, mix 80 µL of the new Donor Solution with 120 µL of PBS.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
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Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
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