The High, Medium, and Low Permeability Controls are 10mM stock solutions of Chloramphenicol, Diclofenac, and Theophylline, respectively, in DMSO.
Pipetting devices, DMSO, PBS, UV Plates (Cat # P96UV), and an absorbance plate reader capable of absorbance spectra.
The donor plate membrane is a Hydrophobic PVDF membrane with a pore size of 0.45 µm.
We recommend against using higher concentrations of DMSO in the PAMPA assay. The spectrophotometric background in the Acceptor solution will increase with higher DMSO concentrations, and the higher concentration of DMSO may alter the membrane and affect the result.
If your compound(s) is/are insoluble in PBS with 5% DMSO, clarify the solution by centrifugation at 13,000xg for 10 minutes then use the supernatant for the Donor solution. For the Equilibrium Standard, mix 80 µL of the new Donor Solution with 120 µL of PBS.
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