The High, Medium, and Low Permeability Controls are 10mM stock solutions of Chloramphenicol, Diclofenac, and Theophylline, respectively, in DMSO.
Pipetting devices, DMSO, PBS, UV Plates (Cat # P96UV), and an absorbance plate reader capable of absorbance spectra.
The donor plate membrane is a Hydrophobic PVDF membrane with a pore size of 0.45 µm.
We recommend against using higher concentrations of DMSO in the PAMPA assay. The spectrophotometric background in the Acceptor solution will increase with higher DMSO concentrations, and the higher concentration of DMSO may alter the membrane and affect the result.
If your compound(s) is/are insoluble in PBS with 5% DMSO, clarify the solution by centrifugation at 13,000xg for 10 minutes then use the supernatant for the Donor solution. For the Equilibrium Standard, mix 80 µL of the new Donor Solution with 120 µL of PBS.
No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Quality and User-friendly • Expert Technical Support • Competitive Prices • Expansive Catalogue • Trusted Globally