EnzyChrom™ Nitric Oxide Synthase Assay Kit

Protocol SDS

Application

• For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.

Key Features

• Sensitive and accurate. Detection range 0.25 – 25 U/L in a 96-well plate.
• Rapid and reliable. Can be completed in 40 min if the reduction of NO₃^– to NO₂^– is performed at 60°C.

Method

• OD540nm

Samples

• Biological

Species

• All

Procedure

• 40 min

Size

• 100 tests

Detection Limit

• 0.25 U/L

Shelf Life

• 6 months

More Details

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins, and direct covalent interaction with functional biomolecules. Simple, direct, and non-radioactive procedures for measuring NOS are becoming popular in Research and Drug Discovery. BioAssay Systems EnzyChrom™ Nitric Oxide Synthase Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following the reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 min.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Macit, C et al (2020). The effects of calorie restriction and exercise on age-related alterations in corpus cavernosum. Frontiers in Physiology, 11. Assay: Nitric Oxide Synthase in rat tissue.

Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Nitric oxide synthase in mice brain tissue.

Kimura, K. et al (2016). Pleiotropic effects of linagliptin monotherapy on levels of nitric oxide, nitric oxide synthase, and superoxide dismutase in hemodialysis patients with diabetes. The Showa University Journal of Medical Sciences, 28(1), 9-17. Assay: Nitric oxide synthase in angus steer serum.

Gehman, MA (2015). The role of BATF2 in LPS/IFNgamma polarized macrophages. Theses and Dissertations-Microbiology, Immunology, and Molecular Genetics 10: 1-205. Assay: Nitric oxide synthase in mice macrophages.

Hiba S et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Toxicological & Environmental Chemistry 96(2): 307-317. Assay: Nitric oxide synthase in rat tissue.

Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Tox. & Environ. Chem. 96(2):307-317 Assay: Nitric oxide synthase in rat tissue.

Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Tox. & Environ. Chem. 96(2):307-317 Assay: Nitric Oxide Synthase in rat tissue.

Meng, JP (2013). Hydrogen sulfide promotes nitric oxide production in corpus cavernosum by enhancing expression of endothelial nitric oxide synthase. International journal of impotence research 25.3: 86-90. Assay: Nitric oxide synthase in rat Corpus Cavernosum Tissue.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.