Urine samples should be diluted at least 5-fold with dH2O and an internal standard should be used.
Samples will need three separate reactions:
1) sample plus standard
2) sample alone
3) sample blank.
For the internal standard prepare 200 µL 1250 µM standard by mixing 125 µL 2 mM Standard and 75 µL dH2O.
For the sample plus standard well, add 5 µL 1250 µM standard and 20 µL sample.
For the sample and sample blank wells, add 5 µL dH2O and 20 µL sample.
Prepare enough Working Reagent (WR) for all assay wells by mixing, for each well, 85 µL Assay Buffer, 1 µL LAA Enzyme, 1 µL HRP Enzyme, 1 µL Dye Reagent. Prepare a WR without the LAA Enzyme for the sample blank well(s).
Add 80 µL of the WR to each well. Tap plate briefly to mix.
Incubate at room temperature for 60 min. Use a plate reader to read OD570nm.
The sample L-amino acid concentration is computed as follows:
where RSAMPLE, RBLANK, and RSTANDARD are the OD or fluorescence values of the Sample, Sample Blank, and the Sample plus Standard respectively. n is the sample dilution factor. Note: The volume of the internal standard is 4× lower than the sample volume; thus, the internal standard concentration should be divided by 4.
This kit has been tested on serum, culture media, mammalian cell lysates, urine, and solid food samples (e.g. ground beef).
Yes, unused reagents can be stored according to the assay protocol.
No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.
Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Technical support provided by the very scientists that develop the assays.
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Because we develop and manufacture the products, our prices are lower than competitors on the market
With over 200 different products, acquire all your assay kit needs in one order.
Products used by clients worldwide with distributors in over 60 countries.
BioAssay Systems is committed to producing innovative, high-quality and cost-effective products and to providing expert technical service to our valued customers.