Protocol SDS

Application

• For quantitative determination of indole and evaluation of drug effects on its metabolism.

Key Features

• Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately.

Method

• OD565nm

Samples

• Bacterial growth media and other biological samples

Species

• All

Procedure

• Under 5 min

Size

• 100 tests

Detection Limit

• 3 µM

Shelf Life

• 6 months

More Details

INDOLE is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole. BioAssay Systems indole assay kit is based on a modified version of Ehlrich’s and Kovac’s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.