This kit is shipped on ice. Upon receiving it, please keep the kit in the freezer (-20°C).
Due to high levels of interference in urine samples, the assay cannot be used to detect tryptophan in urine.
Yes, there is a standard curve in the datasheet; we include this to show the linear detection range of the kit for quantifying tryptophan. Due to interference matrices in serum and plasma, an internal standard must be run. The datasheet has detailed information on how to perform this and calculate tryptophan concentration from it.
It should be noted that this standard curve will not be accurate for determining tryptophan concentration in serum or plasma samples. However, if you wish to construct a standard curve, you may dilute the Tryptophan Standard in dH2O to concentrations within the kit’s detection range (e.g. 100µM, 50µM, 25µM, 0µM) and use these as samples for the fluorimetric (10µL) procedure. Add 100µL Working Reagents to these samples, incubate for 60 minutes, and read the RFU.
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagent should be made fresh for each assay.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
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