QuantiChrom™ Indican Assay Kit

Application

For quantitative determination of indican (indoxyl sulfate) in urine samples.

Key Features

Fast and sensitive. Linear detection range: 0.2 – 20 mg/dL (8-800 µmoles/L).
Fast and high-throughput. Homogeneous “mix-incubate-measure” type assay. The assay takes only 10 min. Can be readily automated for processing thousands of samples per day.

Method

OD480nm

Samples

Urine

Species

Procedure

10 min

Size

1000 tests

Detection Limit

0.2 mg/dL

Shelf Life

12 months

More Details

Indican (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of dietary tryptophan to indole in the small intestine. A large body of evidence supports the use of urinary indican testing as a beneficial point-of-care screening tool for dysbiosis, small intestinal bacterial overgrowth (SIBO), constipation, malabsorption, intestinal mucosal permeability etc. Common microorganisms that contribute to positive indican tests include Salmonella, Shigella, Campylobacter jejuni, yersinia enterocolitica, Citrobacter freundi, Citrobacter diversus, Klebsiella pneumoniae, Pseudomonas aeruginosa, certain strains of Escherichia coli, Staphyloccocus aureus, some strains of bacteriodes, Clostridium difficile, Candida albicans, other candida species etc. Traditional Indican test using Obermayer’s reagent gives only qualitative results. BioAssay Systems’ quantitative indican assay kit is based on an improved Curzon and Walsh method. In this assay, indican reacts with chromogen. The color intensity of the product at 480nm is directly proportional to the indican concentration in the sample.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Fukuchi M, et al. (2020). Effect of Lactic Acid Bacteria-Fermented Soy Milk Extract (LEX) on Urinary 3-Indoxyl Sulfate in Japanese Healthy Adult Women: An Open-Label Pilot Study.Nutrition and Dietary Supplements. 12: 301-309. Assay: Indican in human urine.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

$439.00

Catalog No: DIDC-01K Category : Blood & Urine Chemistry Tags Blood & Urine Chemistry, DIDC-01K, Indican assay kits, Indican determination kits, Indican measurement kits, Indican test kits, QuantiChrom Indican Assay Kits, QuantiChromTM Indican Assay Kit

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt