QuantiFluo™ HRP Reagent

Protocol SDS

Application

• Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530/585 nm), pink-colored product upon reaction with a peroxidase. The fluorescent product can also be visualized under a light source (510-550 nm).

Key Features

• For both absorbance or fluorescence detection.
• Highly sensitive.
• Convenient and fast.

Method

• OD570nm, FL530/585nm

Samples

• Biological

Species

• All

Procedure

• 30 min

Size

• 500 tests

Shelf Life

• 12 months

More Details

QuantiFluo™ Horseradish Peroxidase Reagent is a stable 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent formulated for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent product (λex/em = 530/585 nm) when reacted with H₂O₂ by a peroxidase. The fluorescent product can also be visualized under a light source (530 ± 20 nm).

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.