Fish samples: Ahi tuna, Mahi Mahi, Albacore, Mackerel, Anchovy, Salmon, Tilapia. Red wine, white wine, and milk.
The lower detection limit of the strips for seafood, red wine, and milk samples is 20 ppm.
Very dark purple indicates that the diluted sample concentration is greater than 200 ppm. To obtain a more accurate concentration, the sample should be diluted and retested. Multiply the concentration of the corresponding color by the dilution factor to determine the histamine concentration.
Fish samples can be mashed with a tooth pick and then vigorously shaken for 2 minutes in the extraction buffer.
Milk samples require a 2× Dilution. Carefully add 200 µL of Extraction Buffer to a Sample tube. Then, add 200 µL of milk. Replace the cap, securely close the vial, and invert the vial a couple of times to mix the diluted sample. Proceed with step 1 of the test procedure on the product protocol. Dilution Factor=2
Histamine can be measured in white wine samples, but please note that only levels greater than 20 ppm can be detected and white wine rarely has levels of histamine this high. White wine samples should be diluted 2× in Extraction buffer. See question 5 for instructions.
Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, preferably cool location protected from light. For long term storage (>30 days), we recommend keeping the Extraction Buffer at 4°C or below.
Most red wine histamine levels are 60-3,800 µg/L (or 0.06-3.8 ppm). These levels may cause a slight color change, but it would be difficult to perceive. Therefore most normal wines have histamine levels below the detection limit of 20 ppm. These strips are better suited for identifying an issue with the wine, for example, if there is a problem with the fermentation, the wine may have elevated histamine levels that can be seen with the test strips.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: USPS
Delivery: 2-6 days (US), 6-15 days (intl)
Storage: RT upon receipt
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