QuantiChrom™ Glutathione S-transferase Assay Kit

Protocol SDS

Application

• For quantitative determination of glutathione S-transferase activity and evaluation of drug modulators.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 2 to 80 U/L for 10 min reaction at 25°C.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD340nm

Samples

• Biological samples (e.g. cell lysates, tissues, etc.)

Species

• All

Procedure

• 10 min

Size

• 100 tests

Detection Limit

• 2 U/L

Shelf Life

• 6 months

More Details

GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compound’s electrophilic and/or hydrophobic sites.BioAssay Systems Glutathione S-transferase assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.

How do I store the kit?

This kit is shipped at ambient temperature. Upon receiving, please keep the kit at 4°C. Store reconstituted glutathione tube at -20°C and use within 3 weeks.

What kind of samples can be used?

This kit can be used in cell, tissue, plasma, and erythrocyte samples, etc.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. Since glutathione is quite unstable after reconstitution, store unused glutathione at -20°C and avoid repeated freeze/thaw cycles.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.