QuantiChrom™ Glucose Dehydrogenase Assay Kit

Application

• For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 200 U/L for 15 min reaction.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples (e.g. plasma, serum, tissue, and culture media)

Species

• All

Procedure

• 15 min

Size

• 100 tests

Detection Limit

• 0.5 U/L

Shelf Life

• 6 months

More Details

• GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donors with other acceptors. GDH participates in the pentose phosphate pathway. BioAssay Systems’ non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.