Yes, serum and plasma samples should be diluted 10-fold in dH2O prior assay. Correct for dilution factor (n = 10) in the calculation.
Yes, urine samples should be diluted 20-fold in dH2O prior assay. Correct for dilution factor (n = 20) in the calculation.
Samples with oxidants or ROS will yield lower measurements of antioxidant potential because antioxidants in the sample will reduce the oxidants and ROS rather than the Fe3+, this results in lower levels of Fe2+,which is what the assay measures to determine FRAP.
This assay measures antioxidant potential to reduce Fe3+ to Fe2+. If the sample has intrinsic Fe2+, this will yield a false positive for antioxidant potential.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
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Please email or call 1-510-782-9988 x 2 to request assay service.
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Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: 4°C upon receipt
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