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EnzyFluo™ Elastase Inhibitor Assay Kit
Application
- For evaluation of drugs and screening of potential inhibitors to elastase proteases.
Key Features
- Safe. Non-radioactive assay.
- Homogeneous and convenient. Homogenous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and high-throughput. Can be readily automated to assay thousands of samples per day. Robust assay with a Z' factor of > 0.8.
Method
- FL485/530nm
Samples
- Compounds that affect elastase enzyme activity.
Species
- All
Procedures
- 45 min
Size
- 100 tests (Custom bulk sizes available upon request)
Shelf Life
- 6 months
Screening Services
- BioAssay Systems offers comprehensive lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us.
More Details
PANCREATIC ELASTASE (PE, EC 3.4.21.36) and NEUTROPHIL ELASTASE (NE, EC 3.4.21.37) are serine proteases that exhibit similar elastin-degrading functions in different physiological roles. Neutrophil elastase is produced as an immune response to inflammation or infection, while pancreatic elastase hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. BioAssay Systems' EnzyFluo™ Elastase Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate to quantify elastase activity in serine proteases. The fluorescein label is highly quenched. Upon digestion by elastase present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.What is the source of the Elastase in this kit?
The provided enzyme is Human Neutrophil Elastase; however, this assay has also been validated with porcine elastase (MedChem, cat# HY-P2974) if desired.
What is the control inhibitor made up of?
The control inhibitor is a 200 mM solution of Phenylmethylsulfonyl fluoride (PMSF).
Can the substrate be refrozen after use?
While the substrate can handle a few freeze-thaw cycles, if the customer plans to run many tests, it would be best to aliquot the substrate into light-protected tubes and thaw as needed.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$499.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.
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