SuperLight™ Dual Luciferase Reporter Gene Assay Kit

Protocol SDS

Application

• Bioluminescent reagent system for rapid quantitation of firefly and Ranilla luciferase reporter gene expression in transfected cells.

Key Features

• High sensitivity and wide detection range. Detection of as little as 2 fg luciferase.
• Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, and measured with any luminometer. Can be readily automated on HTS liquid handling systems.
• Fast and convenient. Three-step assay allows the detection of dual luciferase levels within 20 minutes.

Method

• Luminescence

Samples

• Cells etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 2 fg luciferase

Shelf Life

• 12 months

More Details

The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The SuperLight™ Dual-Luciferase Reporter Gene Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by the addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell-free transcription/translation reactions. Assays can be performed in tubes, cuvettes, or multi-well plates.