EnzyFluo™ D-Lactate Assay Kit

Protocol SDS

Application

• For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM D-lactate in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• FL530/585 nm

Samples

• Serum, plasma, cell culture media etc

Species

• All

Procedure

• 60 min

Size

• 100 tests

Detection Limit

• 1 µM

Shelf Life

• 6 months

More Details

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Palomino, Nahui, et al (2017). Vaginal Lactobacillus inhibits HIV-1 replication in human tissues ex vivo. Frontiers in microbiology 8: 906. Assay: D-Lactate in Lactobacillus culture.

Saville, Renee M., et al (2016). Compositions and methods for biological production of lactate from C1 compounds using lactate dehydrogenase transformants. U.S. Patent Application No. 14/898,948. Assay: D-Lactate in microorganism culture.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.