EnzyChrom™ Choline Assay Kit

Protocol SDS

Application

• For quantitative determination of choline and evaluation of drug effects on choline metabolism.

Key Features

• Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline.

Method

• OD570nm, or FL530/585nm

Samples

• Serum, plasma, urine, saliva, milk, tissue, cells etc

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• OD, FL: 1, 0.2 µM

Shelf Life

• 6 months

More Details

CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illnesses such as anxiety. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H₂O₂ which reacts with a specific dye to form a pink-colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.

What are known interferences?

Chondroitin sulfate C (chondroitin-6-sulfate) appears to interfere with the assay; creating false positive results.

Winnard Jr, PT et al (2020). Brain metabolites in cholinergic and glutamatergic pathways are altered by pancreatic cancer cachexia. Journal of Cachexia, Sarcopenia and Muscle, 11(6), Assay: Choline in mouse plasma.

Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.

Benedito-Palos, L et al (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Choline in fish plasma.

Ballester-Lozano, GF et al (2014). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.

Strilakou, AA et al (2014). Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats. Canadian Journal of Physiology and Pharmacology 92(1): 78-84. Assay: Choline in rat serum.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.