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QuantiChrom™ β-Glucuronidase Assay Kit


  • For quantitative determination of β-glucuronidase enzyme activity in biological samples, e.g. blood, tissue and bacterial samples.

Key Features

  • Highly Sensitive. The fluorimetric nature of the kit detects 1×10-4 U/L to 8 U/L BG activity in 96 well plate with only 10 µL sample within 30 min.
  • Versatile. This assay has been validated to work with both animal and E. coli derived β-glucuronidase.
  • High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.


  • FL365/450nm


  • Blood, tissue and bacterial samples


  • All


  • 30 min


  • 100 tests in 96-well plate

Detection Limit

  • 10-4 U/L

Shelf Life

  • 6 months

More Details

BETA-GLUCURONIDASE (BG) is an enzyme which catalyzes the hydrolysis of β-glucuronide bonds. In humans, β-glucuronidase catalyzes the hydrolysis of β-D-glucuronic acids from glycoproteins and mucopolysaccharides such as heparan sulfate. β-glucuronidase activity is important for drug metabolism as drugs are often conjugated to glucuronic acid to make for a more water soluble delivery system, relying on subsequent β-glucuronidase activity to release the active drug. Bilirubin clearance is also facilitated by glucuronidation. GUSB is often used as a reporter gene to track gene expression through β-glucuronidase activity. BioAssay Systems’ fluorimetric BG assay uses a β-D-glucuronide substrate which fluoresces upon hydrolysis with β-glucuronidase and is read at 365/450 nm.

Weight 1 lbs
Dimensions 6 × 4 × 3 in


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