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SuperLight™ Atto HRP Reagent



Application

  • For HRP determination in ELISA, Southern Blot, Western Blot.

Key Features

  • Single reagent: Single, stable, and ready to use.
  • High sensitivity: Detection of HRP in the atto- to low femtogram range.

Method

  • Luminescence

Samples

  • horse-radish peroxidase and its conjugates

Species

  • All

Procedures

  • 10 min

Size

  • 200 tests in 96-well plate (800 tests in 384-well plate)

Detection Limit

  • Atto- to low femtomolar

Shelf Life

  • 6 months

More Details

  • PEROXIDASES (or peroxide reductases, EC number 1.11.1.x) are a large group of enzymes that break up peroxides and play important roles in various biological processes. Horse Radish Peroxidase (HRP) is an enzyme commonly used for conjugation. One example is antibody-HRP conjugate that is frequently and widely used in immunoassays such as ELISA and Western blots. BioAssay Systems’ SuperLight™ Atto HRP Reagent provides an ultra-sensitive luminescent means for detecting HRP activity and its conjugates. The improved reagent rapidly reacts with peroxidase or the HRP in an HRP-conjugate, producing highly stable and intense luminescence for maximum detection of the peroxidase activity in solution assays.

What are the applications of this reagent?

This ultra-sensitive reagent is used to detect peroxidase activity (e.g. horse-radish peroxidase) and its conjugates in ELISA or Western blots.

What equipment should I use?

You can use any luminometer, such as Molecular Devices, Perkin Elmer readers.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

SLAPfig
$99.00
- +

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

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