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Ammonia Assay

Accurate Ammonia Results Across the Research You Do

Your work demands data you can trust. BioAssay Systems ammonia assays are cited across modern research—from cell-culture and biomaterials studies to translational physiology, ag/animal science, and plasma-chemistry workflows—because they’re built for specificity, speed, and plate-reader simplicity. That’s why researchers reach for EnzyChrom™ ENH3-100 to quantify ammonia in cell-biology and biomaterial studies, and choose QuantiFluo™ kits for high-throughput urine and plasma measurements when reliable numbers matter.

Ammonia Assays

Choose the Kit That Fits Your Workflow

EnzyChrom™ Ammonia/Ammonium Assay Kit

ENH3-100

BEST FOR

Routine biofluids with enzyme specificity and instrument flexibility

Readout:

Absorbance 340 nm or fluorescence (Ex/Em 360/450 nm)—same kit

Chemistry:

GDH/NADH enzymatic method for ammonia specificity

Typical time:

~30 minutes; stated linear range: ~24–1000 μM

Sample Types:

Serum, plasma, urine, saliva, cell-culture media (typical dilutions as needed)

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QuantiFluo™
Ammonia Assay Kit

DNH3-200

BEST FOR

Speed and high-throughput in cleaner/low-amine matrices

Readout:

Fluorescence (Ex/Em 360/450 nm)

Typical time:

~15 minutes; HTS-automatable

Stated range/LOD:

~0.012–1 mM; ~12 μM LOD

Notes:

OPA-based chemistry; for complex biological samples, pair with deproteinization/amine removal for best specificity

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QuantiFluo™
Ammonia Assay Kit II

D2NH3-100

BEST FOR

Fluorescence-only simplicity with the broadest working range

Readout:

Fluorescence (Ex/Em 415/475 nm)

Workflow:

Single-component reagent; ~20 minutes; HTS-friendly

Stated Linear Range:

0.005–2 mM

Sample Types:

Compatible with biological and environmental samples using practical dilutions

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