QuantiFluo™ Alkaline Phosphatase Assay Kit

Protocol SDS

Application

• For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate.

Key Features

• High sensitivity and wide linear range. Use 10 µL sample. The detection limit of 0.02 U/L (20 min reaction).
• Homogeneous and simple procedure. A simple “mix-and-measure” procedure allows reliable quantitation of ALP activity within 20 minutes.
• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Method

• FL360/450nm

Samples

• Serum, plasma etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 0.02 U/L

Shelf Life

• 2 years

More Details

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This improved method utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into a highly fluorescent product 4-methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Domazetovic, V., et al (2020). Blueberry juice antioxidants protect osteogenic activity against oxidative stress and improve long-term activation of the mineralization process in human osteoblast-like saos-2 cells: Involvement of sirt1. Antioxidants (Basel, Switzerland), 9(2). Assay: Alkaline Phosphatase in human bone cells.

Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture. 454 (2016) 8-18. Assay: Alkaline phosphatase in fish plasma.

Maryam, RR et al. (2015). The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs). Arch Oral Biol. 60(4): 546-556. Assay: Alkaline phosphatase in rat cells.

Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: alkaline phosphatase enzyme activity in fish plasma.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.