Shopping Cart

QuantiFluo™ Chymotrypsin Inhibitor Assay Kit

Description



Application

  • For evaluation of drugs and screening of potential inhibitors to chymotrypsin proteases.

Key Features

  • Safe. Non-radioactive assay.
  • Homogeneous and convenient. Homogenous “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
  • Robust and High-throughput. Can be readily automated to assay thousands of samples per day. Robust assay with a Z’ factor of >0.8.

Method

  • FL485/530 nm

Samples

  • Compounds that affect chymotrypsin protease activity.

Species

  • All

Procedures

  • 45 min

Size

  • 100 tests in 96-well plate.

Detection Limit

  • NA

Shelf Life

  • 6 months

More Details

CHYMOTRYPSIN (EC 3.4.21.1) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Chymotrypsin predominantly cleaves peptide chains at the carboxyl side of the aromatic phenylalanine, tryptophan, and tyrosine amino acids. BioAssay System’s QuantiFluo™ Chymotrypsin Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate. The fluorescein label is highly quenched. Upon digestion by chymotrypsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.

Additional information

Weight1 lbs
Dimensions6 × 4 × 3 in

QuantiFluo™ Chymotrypsin Inhibitor Assay Kit



Application

  • For evaluation of drugs and screening of potential inhibitors to chymotrypsin proteases.

Key Features

  • Safe. Non-radioactive assay.
  • Homogeneous and convenient. Homogenous “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
  • Robust and High-throughput. Can be readily automated to assay thousands of samples per day. Robust assay with a Z’ factor of >0.8.

Method

  • FL485/530 nm

Samples

  • Compounds that affect chymotrypsin protease activity.

Species

  • All

Procedures

  • 45 min

Size

  • 100 tests in 96-well plate.

Detection Limit

  • NA

Shelf Life

  • 6 months

More Details

CHYMOTRYPSIN (EC 3.4.21.1) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Chymotrypsin predominantly cleaves peptide chains at the carboxyl side of the aromatic phenylalanine, tryptophan, and tyrosine amino acids. BioAssay System’s QuantiFluo™ Chymotrypsin Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate. The fluorescein label is highly quenched. Upon digestion by chymotrypsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.
Chymotrypsin Inhibitor Assay Kit
$439.00

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

Why BioAssay Systems

Quality and User-friendly • Expert Technical Support • Competitive Prices • Expansive Catalogue • Trusted Globally