Description
Application
- For determination of inosine concentration in cell lysate, serum, and other biological samples.
Key Features
- Sensitive and accurate. Use as little as 20 μL of sample. Linear detection range in 96-well plate for 30-minute incubation with 1 to 25 μM.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
- Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day.
Method
- FL530/585nm
Samples
- Biological samples
Species
- All
Procedures
- 30 min
Size
- 100 tests in 96-well plate
Detection Limit
- 1 μM
Shelf Life
- 6 months
More Details
INOSINE is a purine nucleoside formed from Ribose and Hypoxanthine that can be found in most animal tissues and fluids. Inosine is most often found in tRNAs and is important for proper translation of the genetic code. It may play a role in cancer immunometabolism. It is degraded via the purine degradation pathway, first by purine nucleoside phosphorylase to hypoxanthine, then to xanthine by xanthine oxidase and then finally to uric acid by further action of xanthine oxidase.
Simple, direct and high-throughput assays for measuring inosine find wide applications in research and drug discovery. BioAssay Systems’ inosine assay kit uses a single Working Reagent that combines the enzyme reactions and color reaction in one step. The change in fluorescence intensity at λex/em = 530/585 nm is directly proportional to inosine concentration in the sample.
