Description
Application
- For quantitative determination of αKG in serum, tissue and cell lysates.
Key Features
- Sensitive and accurate. Linear detection range in 96-well plate: 1.3 to 200 μM for colorimetric assays and 0.08 to 20 μM for fluorimetric assays.
- Fast and convenient. Room temperature assay with a 30 min incubation. No 37°C incubator is needed.
- High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Method
- OD570nm or FL530/585nm
Samples
- Serum, tissue and cell lysate
Species
- All
Procedures
- 30 min
Size
- 100 tests in 96-well plate
Detection Limit
- 1.3 μM for OD, 0.08 μM for FL
Shelf Life
- 6 months
More Details
α-Ketoglutarate (αKG) is a crucial intermediate in the citric acid (TCA) cycle and plays an important role in amino acid formation, nitrogen transport, and oxidation reactions. αKG can modulate muscle and bone growth as well as the aging process, and has also been investigated for its potential to enhance anticancer immune functions. BioAssay Systems’ α-ketoglutarate assay uses a single Working Reagent that combines αKG deamination, pyruvate oxidation and hydrogen peroxide determination. The change in OD570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the αKG present in the sample.
