Nitric Oxide Synthase Inhibitor Screening Service
Nitric Oxide Synthase (220.127.116.11) catalyzes the formation of nitric oxide, a reactive radical, which plays an important role in many key physiological functions such as synaptic plasticity in the CNS and blood pressure regulation. There are three eukaryotic Nitric Oxide Synthase enzymes – nNOS (NOS-1), iNOS (NOS-2), and eNOS (NOS-3). All three enzymes possess FAD, FMN, Protoporphyrin IX (Heme), and Tetrahydrobiopterin cofactors. In addition, Calmodulin is closely associated with each enzyme. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system.
DPI IC50 Determination
To test the suitability of BioAssay System’s Nitric Oxide Synthase Assay for screening for inhibitors, we performed an initial experiment with a known inducible Nitric Oxide Synthase inhibitor, Diphenyleneiodonium chloride (DPI), with murine iNOS. We observed an IC50 of 64.8 nM for DPI with murine iNOS. The percent enzyme activity was plotted versus the concentration of DPI using Prism 4 (GraphPad Software Inc.).
- High-throughtput. Assays are carried out in 96-well plate.
- Robust. A Z’ factor of up to 0.95 is possible.
- The assay tolerates up to 5% DMSO.
- NOS inhibitor hit profiling.
- NOS inhibitor lead generation.
- Structure-activity relationship studies.