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Nitric Oxide Synthase Inhibitor Screening Service
Summary
Nitric Oxide Synthase (1.14.13.39) catalyzes the formation of nitric oxide, a reactive radical, which plays an important role in many key physiological functions such as synaptic plasticity in the CNS and blood pressure regulation. There are three eukaryotic Nitric Oxide Synthase enzymes – nNOS (NOS-1), iNOS (NOS-2), and eNOS (NOS-3). All three enzymes possess FAD, FMN, Protoporphyrin IX (Heme), and Tetrahydrobiopterin cofactors. In addition, Calmodulin is closely associated with each enzyme. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system.
DPI IC50 Determination
To test the suitability of BioAssay System’s Nitric Oxide Synthase Assay for screening for inhibitors, we performed an initial experiment with a known inducible Nitric Oxide Synthase inhibitor, Diphenyleneiodonium chloride (DPI), with murine iNOS. We observed an IC50 of 64.8 nM for DPI with murine iNOS. The percent enzyme activity was plotted versus the concentration of DPI using Prism 4 (GraphPad Software Inc.).
Key Features
- High-throughtput. Assays are carried out in 96-well plate.
- Robust. A Z’ factor of up to 0.95 is possible.
- The assay tolerates up to 5% DMSO.
- NOS inhibitor hit profiling.
- NOS inhibitor lead generation.
- Structure-activity relationship studies.
Customer Testimonials
"The guys at BioAssay Systems know their science! I had to develop a bioassay for our company and Robert was very quick to respond to keep us up to date. They are knowledgeable and very resourceful. They directed us to one of the bulk suppliers that could give us a really good deal on our active ingredient. They keep very detailed records for our research needs. I would recommend working with BioAssay Systems on your assay development projects!"
"It is a pleasure working together with BioAssay Systems. I brought samples to their Hayward location and received the results in less than 24 hr. The discussion with Robert Z was courteous, open, and with a depth of knowledge. Robert has a PhD from Stanford. Also, Frank Huang, the CEO, had sufficient scientific curiosity to try out our cleanser and provide feedback! This is beyond the call of customer relations. BioAssay Systems is a company with a lot of expertise and a friendly, responsive approach to problem solving. I will enthusiastically continue to work with them to carry out our research."
"I would like to show my appreciation to the Bioassay Systems service team. We are developing an assay to measure the change in lactate concentration of single cell samples using fluorescent microscopy. The BioAssay Systems team was quite helpful in assisting us to optimize their fluorescent L-Lactate assay kit for our unique experimental set-up. The BioAssay Systems team is always friendly and knowledgeable. We know that we can rely on them for fast and professional service."
"While developing biodegradable coatings for Drug Eluting Stents I worked with Bob to develop an accurate, precise and sensitive assay for determining the amount of polymer remaining in tissue. It was a pleasure to work with Bob and his team, they are very thorough, they understand the science very well and they always deliver on time and at the right price. I would highly recommend anyone using BioAssay Systems services in the future."
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