QuantiChrom™ Formaldehyde Assay Kit

Application

• For quantitative and direct determination of formaldehyde concentrations in biological, food, and environmental samples.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. As low as 1.5 µM (45 ppb) formaldehyde can be quantified.
• Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL370/470nm

Samples

• Biological, food, beverage, environment

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 1.5 µM (45 ppb)

Shelf Life

• 18 months

More Details

What samples have you tested?

This kit has been tested in blood and urine samples.

Can I use whole blood samples?

We have done some tests in whole blood samples. In the internal standard method, the fluorescence intensity is greatly quenched so the sensitivity will be reduced by ~70X (detection limit to about 105 μM, way above typical formaldehyde concentrations in blood samples). As a result, it is not suitable for whole blood samples.

Below is a revised protocol for whole blood treatment and we have verified it works well. It is simple and quick. 100 μL blood is needed. For whole blood samples include the following revised deproteination protocol:

– Add 100 μL 10% TCA per 100 μL whole blood.
– Vortex and centrifuge 10 min at 14000 rpm
– Transfer 100 μL supernatant to clean tube and add 25 μL Neutralizer.
– Vortex and add 50 μL to 2 separate wells (1 well for full WR and one for Blank WR)
– Follow protocol, run a standard curve in dH₂O. Read fluorescence for Sample, Sample Blank and the standards.
– Subtract RFU of Blank from sample RFU and multiply by 2.5 (dilution factor for deproteination). Divide corrected Sample ΔRFU by slope of water (H₂O) standard curve.

This method can detect as low as 1.5 μM formaldehyde (same as in H₂O).

What is the benefit of using an internal standard?

The use of an internal standard can correct for potential interference by sample matrix.

Will the assay kit work in a 384 well plate, cuvette, etc?

Yes, the assay can be used in any standard plate and cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 μL, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 μL).

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay.

Do I need to use a standard or standard curve with each assay run?

Yes, it is highly recommended.

Will the assay react with other aldehydes?

No, the assay is specific for formaldehyde and will not react with other aldehydes.

Ai, L.,et al. (2019). Endogenous formaldehyde is a memory-related molecule in mice and humans. Communications Biology, 2(1), 446. Assay: Formaldehyde in mouse tissue.

Zhai, R et al (2016). Evidence for Conversion of Methanol to Formaldehyde in Nonhuman Primate Brain. Analylatical Cell Pathology (Amsterdam): 4598454. Assay: Formaldehyde in monkey CSF.

Yang, M et al (2014). Alzheimer’s disease and methanol toxicity (part 2): lessons from four rhesus macaques (Macaca mulatta) chronically fed methanol. J Alzheimers Dis. 41(4): 1131-1147. Assay: Formaldehyde in monkey whole blood.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.