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In order to test the suitability of BioAssay Systems Arginase Assay for screening for arginase inhibitors, we performed some initial experiments to determine the Km of L-arginine for a purified human arginase and the IC50 for a known arginase inhibitor, ABH. We measured a Km of 3.4 ± 0.2 mM. Using 3 mM L-arginine, we observed an IC50 = 0.14 ± 0.1 µM for ABH. We also verified that 2 v% DMSO will not affect the assay.
|2 v% DMSO||0.3995||1.4476|
|0 v% DMSO||0.3978||1.4181|