Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.
Yes, the assay can be used in any standard plate and cuvette. Simply adjust the total reaction volume to your container. For 384 well use 50 μL, for a cuvette use 500 μL or 1 mL.
Yes, this assay works in all species.
Please refer to the protocol for sample preparation:
Blood samples: Serum and plasma should be diluted 2-5 fold.
Tissue: Prior to dissection, rinse tissue in Tris buffered saline (pH 7.4) to remove blood. Homogenize tissue (50 mg) in 200 μL 50 mM Tris buffer (pH 7.5). Centrifuge at 14,000 x g for 10 min at 4°C. Remove supernatant for assay.
Cell lysate: Collect cells by centrifugation at 2,000 x g for 5 min at 4°C. For adherent cells, do not harvest cells using proteolytic enzymes; rather use a rubber policeman. Homogenize or sonicate cells in an appropriate volume of cold 50 mM Tris buffer (pH 7.5), approximately one million cells per mL. Centrifuge at 14,000 x g for 10 min at 4°C. Remove supernatant for assay. All samples can be stored at -80 to -20°C for at least one month.
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Since Assay Buffer is very light sensitive, always keeps buffer in the dark (amber bottle or tube) as much as possible for long term usage.
Yes, it is highly recommended.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
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