Description
Application
- For quantitative determination of ACE-1 activity determination in biological samples.
Key Features
- Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
- Sensitive and accurate. Linear detection range 0.01 – 5 U/L ACE-1 in a 96-well plate assay.
- High-throughput. Can be readily automated to assay thousands of samples per day.
Method
- FL360/485nm
Samples
- Serum, cell or tissue lysate, and other biological samples
Species
- All
Procedures
- 45 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.01 U/L
Shelf Life
- 6 months
More Details
ANGIOTENSIN-CONVERTING ENZYME 1, ACE-1 (EC 3.4.15.1) is a critical enzyme in the renin-angiotensin-aldosterone system (RAAS), which regulates blood pressure, fluid, and electrolyte balance in the body. Found primarily in the lung endothelium and kidney epithelial cells, ACE-1 hydrolyzes angiotensin I, cleaving off the His-Leu dipeptide to produce angiotensin II. Its role in the RAAS system makes it a major target for hypertension and cardiovascular treatments.
BioAssay Systems’ QACE1-100 Kit provides a convenient fluorometric method to measure ACE-1 activity in biological samples. In this assay, ACE-1 hydrolyzes the synthetic substrate, releasing the product. Then, o-phthalaldehyde (OPA) fluorescently labels the product for quantification. The increase in fluorescence at λex/em = 360/485 nm is directly proportional to enzyme activity.
