Description
Application
- For quantitative determination of fatty acyl-CoAs in tissue and cell lysates.
Key Features
- Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.3 to 100 µM fatty acyl-CoA. Internal standard method minimizes potential sample matrix interferences.
- Fast and convenient. Room temperature assay with a 40 min incubation. No 37°C incubator is needed.
- High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Method
- FL530/585nm
Samples
- Tissue and cell lysates
Species
- All
Procedures
- 40 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.3 µM
Shelf Life
- 6 months
More Details
Fatty Acyl-CoAs are a combination of Coenzyme A covalently bound to long chain fatty acids. Fatty acyl-CoAs serve as both a precursor for triglyceride and phospholipids synthesis, and a product of lipid catabolism. Catabolically, they facilitate the transport of fatty acids to the mitochondria for shuttling via the carnitine shuttle and subsequent β-oxidation of the fatty acid. Conversion of fatty acids to fatty acyl-CoAs is necessary for generation of ATP during periods of high energy demand. Elevated fatty acyl-CoA levels may indicate dysregulation of lipid metabolism such as due to insulin resistance. Certain genetic disorders may also affect fatty acyl-CoA levels.
BioAssay Systems’ fluorimetric fatty acyl-CoA assay works using a combination of enzymes that utilize fatty acyl-CoA as a substrate, which is coupled to form a fluorescent product. The resulting fluorescence intensity at λexc/em = 530/585 nm is linear to the fatty acyl-CoA concentration in the sample.
