Description
Application
- For quantitative determination of LAP enzyme activity in biological samples, e.g. cells, tissues, and serum samples.
Key Features
- Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.025 to 250 U/L.
- Fast and convenient. The procedure involves addition of a single working reagent and incubation for 20 min. The assay can be run at room temperature or 37°C.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to assay thousands of samples per day.
Method
- OD405nm
Samples
- Cells, serum, and other biological samples
Species
- All
Procedures
- 20 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.025 U/L
Shelf Life
- 6 months
More Details
LEUCINE AMINOPEPTIDASE (LAP) (E.C 3.4.11.1) is an enzyme commonly found in liver cells and intestinal cells that hydrolyzes leucine from the N-terminus of proteins and polypeptides. The expression of LAP increases in damage and immune response, so increased LAP activity in serum samples can indicate potential liver damage, tumorigenesis, or inflammation.
BioAssay Systems' LAP activity assay provides a convenient colorimetric method to measure LAP activity in biological samples. In this assay, LAP hydrolyzes a dye-linked leucine substrate, resulting in a yellow, colored product, measurable at OD405nm. The increase in absorbance at 405 nm (ΔOD) in 20 minutes is directly proportional to the LAP activity.
