Multiplex Assay Services

BioAssay Systems offers multiplex biomarker analysis using Luminex® xMAP® Technology.

The Luminex® xMAP® technology is based on antibody-precoated magnetic microspheres that are embedded with red and infrared fluorophores at different ratios for each analyte-specified region. An array of analyte-specific microspheres can then be combined in a single well to detect and quantify multiple analytes simultaneously, using a Luminex® MAGPIX™ instrument for analysis.

Please contact us by email or call us at +1-510-782-9988 ext 2 with the list of analytes and species specificity you would like measured in the panel to allow us to put together a personalized quote for the service cost.

See below for lists of recommended analytes for specific research areas:

Bone Metabolism
  • Aggrecan
  • AgRP/ART
  • BMP-2
  • BMP-4
  • BMP-9
  • Dkk-1
  • FGF-23
  • IL-1 beta
  • IL-6
  • Insulin
  • Leptin
  • Osteopontin
  • Osteoprotegerin*
  • SOST
  • TNF-alpha
  • TRANCE / RANK L*


*incompatible together in a panel

Oncology Cancer Biomarker Panel 2**
  • CA125 / MUC16
  • FGF basic/FGF2
  • IL-8 / CXCL8
  • CEACAM-5 / CD66e
  • HE4 / WFDC2
  • Leptin / OB
  • Fas / TNFRSF6 / CD95
  • HGF
  • Fas Ligand / TNFSF6
  • IL-6


Cancer Biomarker Panel 2**
  • alpha-Fetoprotein / AFP
  • Osteopontin/OPN
  • TNF-alpha
  • CA15-3/MUC-1
  • Prolactin
  • TRAIL / TNFSF10
  • MIF
  • SCF / c-kit Ligand
  • VEGF
  • TGF-alpha


**Cancer Biomarker Panel sets are mutually exclusive

Cardiovascular Disease
  • Angiopoietin-2
  • FGF basic / FGF2
  • PlGF
  • BMP-9
  • G-CSF
  • VEGF-A
  • EGF
  • HB-EGF
  • VEGF-C
  • Endoglin / CD105
  • HGF
  • IL-8 / CXCL8
  • FGF acidic / FGF1
  • Leptin / OB

Immunology / Cytokines / Chemokines
  • IFN-alpha
  • IL-2
  • IL-10
  • IFN-gamma
  • IL-3
  • IL-15
  • IL-1 alpha / IL-1F1
  • IL-4
  • IL-33
  • IL-1 beta / IL-1F2
  • IL-6
  • VEGF
  • IL-1ra / IL-1F3
  • IL-7
  • CCL2 / JE / MCP-1
  • CCL11 / Eotaxin
  • IL-8 / CXCL8
  • CCL3 / MIP-1 alpha***
  • CXCL1 / GRO alpha / KC / CINC-1
  • CCL4 / MIP-1 beta
  • CXCL10 / IP-10 / CRG-2
  • CCL5 / RANTES***


***incompatible together in a panel

Kidney Biomarkers
  • ADAMTS13
  • Adiponectin
  • alpha1-Microglobulin
  • beta2-Microglobulin
  • Calbindin D
  • CCL2 / MCP-1
  • Collagen IV alpha1
  • CXCL1 / GRO alpha
  • CXCL10 / IP-10
  • CXCL16
  • Cystatin C
  • DPPIV / CD26****
  • EGF
  • FABP1 / L-FABP
  • Fetuin A / AHSG
  • IL-1 ra*****
  • IL-6
  • IL-10
  • IL-18
  • Kallikrein 3 / PSA
  • Lipocalin-2 / NGAL
  • MMP-9
  • Osteopontin
  • RAGE
  • RBP4
  • Renin
  • Resistin
  • SCF
  • TFF3
  • TIM-1 / KIM-1TIMP-1****
  • TNF-alpha
  • TNF RI*****
  • TWEAK
  • uPAR
  • Uromodulin
  • VCAM-1
  • VEGF-A


****incompatible together in a panel *****incompatible together in a panel

See below for the full list of available analytes for each species:

Available Human Analytes
  • 4-1BB / TNFRSF9 / CD137
  • alpha 2-Macroglobulin
  • ADAMTS13
  • Adiponectin / Acrp30
  • Adiponectin / Acrp30
  • Aggrecan
  • AgRP / ART
  • ALCAM / CD166
  • Aldehyde Dehydrogenase 1-A1 / ALDH1A1
  • alpha 1-Microglobulin
  • alpha-Fetoprotein / AFP
  • Angiogenin
  • Angiopoietin-1
  • Angiopoietin-2
  • Angiopoietin-like Protein 3 / ANGPTL3
  • Angiopoietin-like Protein 4 / ANGPTL4
  • Angiopoietin-like Protein 6 / ANGPTL6
  • APP
  • APRIL / TNFSF13
  • Atrial Natriuretic Peptide / ANP
  • beta 2-Microglobulin
  • B7-H3
  • BAFF / BLyS / TNFSF13B
  • BCMA / TNFRSF17
  • BDNF
  • BDNF
  • BMP-2
  • BMP-4
  • BMP-7
  • BMP-9
  • BMP-10
  • C-Peptide
  • C-Reactive Protein / CRP
  • CA125 / MUC16
  • CA15-3 / MUC-1
  • N-Cadherin
  • Calbindin D
  • Carbonic Anhydrase IX / CA9
  • Cathepsin S
  • CCL1 / I-309 / TCA-3
  • CCL2 / JE / MCP-1
  • CCL2 / JE / MCP-1
  • CCL3 / MIP-1 alpha
  • CCL4 / MIP-1 beta
  • CCL5 / RANTES
  • CCL5 / RANTES
  • CCL7 / MCP-3 / MARC
  • CCL7 / MCP-3 / MARC
  • CCL8 / MCP-2
  • CCL11 / Eotaxin
  • CCL11 / Eotaxin
  • CCL13 / MCP-4
  • CCL14 / HCC-1 / HCC-3
  • CCL15 / MIP-1 delta
  • CCL17 / TARC
  • CCL17 / TARC
  • CCL18 / PARC
  • CCL19 / MIP-3 beta
  • CCL20 / MIP-3 alpha
  • CCL21 / 6Ckine
  • CCL21 / 6Ckine
  • CCL22 / MDC
  • CCL23 / MPIF-1
  • CCL24 / Eotaxin-2 / MPIF-2
  • CCL25 / TECK
  • CCL26 / Eotaxin-3
  • CCL27 / CTACK
  • CCL28
  • CD14
  • CD23 / Fc epsilon RII
  • CD27 / TNFRSF7
  • CD30 / TNFRSF8
  • CD31 / PECAM-1
  • CD31 / PECAM-1
  • CD40 / TNFRSF5
  • CD40 Ligand / TNFSF5
  • CD44
  • CD117 / c-kit
  • CD163
  • CD25 / IL-2R alpha
  • CEACAM-1 / CD66a
  • CEACAM-5 / CD66e
  • Chemerin
  • Chitinase 3-like 1
  • Chitinase 3-like 1
  • Coagulation Factor III / Tissue Factor
  • Coagulation Factor XIV / Protein C
  • Collagen I alpha 1
  • Collagen IV alpha 1
  • Complement Component C2
  • Complement Component C5a
  • Complement Component C9
  • Complement Factor D / Adipsin
  • Contactin-1
  • Cripto
  • CX3CL1 / Fractalkine
  • CX3CL1 / Fractalkine
  • CXCL1 / GRO alpha / KC / CINC-1
  • CXCL1 / GRO alpha / KC / CINC-1
  • CXCL2 / GRO beta / MIP-2 / CINC-3
  • CXCL4 / PF4
  • CXCL5 / ENA-78
  • CXCL5 / ENA-78
  • CXCL6 / GCP-2
  • CXCL7 / NAP-2
  • CXCL9 / MIG
  • CXCL10 / IP-10 / CRG-2
  • CXCL10 / IP-10 / CRG-2
  • CXCL11 / I-TAC
  • CXCL12 / SDF-1
  • CXCL13 / BLC / BCA-1
  • CXCL14 / BRAK
  • CXCL16
  • CXCL16
  • Cystatin C
  • D-dimer
  • DcR3 / TNFRSF6B
  • Dkk-1
  • DPPIV / CD26
  • DR3 / TNFRSF25
  • EGF
  • EMMPRIN / CD147
  • EN-RAGE / S100A12
  • Endocan / ESM-1
  • Endoglin / CD105
  • Endostatin
  • Endothelin-1
  • Enolase 2 / Neuron-specific Enolase
  • ENPP-2 / Autotaxin
  • EpCAM / TROP1
  • EphA2
  • ErbB2 / Her2
  • ErbB3 / Her3
  • ESAM
  • FABP4 / A-FABP
  • Fas / TNFRSF6 / CD95
  • Fas Ligand / TNFSF6
  • Ferritin
  • Fetuin A / AHSG
  • FGF acidic / FGF1
  • FGF basic / FGF2 / bFGF
  • FGF-13
  • FGF-23
  • Fibroblast Activation Protein alpha / FAP
  • Fibronectin
  • Flt-3 Ligand / FLT3L
  • Follistatin-like 1 / FSTL1
  • Follistatin-related Gene Protein / FLRG
  • Furin
  • G-CSF
  • Galectin-1
  • Galectin-3
  • Galectin-3BP / MAC-2BP
  • Galectin-9
  • Gas6
  • GDF-15
  • GDNF
  • GITR / TNFRSF18
  • GM-CSF
  • GM-CSF
  • gp130
  • Granzyme A
  • Granzyme B
  • Growth Hormone
  • HB-EGF
  • HB-EGF
  • HE4 / WFDC2
  • HGF
  • HGF
  • HGFR / c-MET
  • HTRA2 / Omi
  • ICAM-1 / CD54
  • IFN-alpha
  • IFN-beta
  • IFN-gamma
  • IFN-gamma
  • IFN-gamma R1 / CD119
  • IGFBP-1
  • IGFBP-2
  • IGFBP-3
  • IGFBP-4
  • IGFBP-6
  • IGFBP-rp1 / IGFBP-7
  • IL-1 alpha / IL-1F1
  • IL-1 beta / IL-1F2
  • IL-1 beta / IL-1F2
  • IL-1ra / IL-1F3
  • IL-1 RI
  • IL-1 RII
  • IL-2
  • IL-2
  • IL-3
  • IL-4
  • IL-4
  • IL-5
  • IL-6
  • IL-6
  • IL-7
  • IL-7
  • IL-8 / CXCL8
  • IL-8 / CXCL8
  • IL-10
  • IL-11
  • IL-12 / IL-23 p40
  • IL-12 p70
  • IL-12 p70
  • IL-13
  • IL-13
  • IL-15
  • IL-15
  • IL-16
  • IL-17 / IL-17A
  • IL-17C
  • IL-17E / IL-25
  • IL-18 / IL-1F4
  • IL-18 / IL-1F4
  • IL-19
  • IL-21
  • IL-23
  • IL-27
  • IL-28A / IFN-lambda 2
  • IL-28B / IFN-lambda 3
  • IL-31
  • IL-33
  • IL-34
  • IL-34
  • IL-36 beta / IL-1F8
  • IL-4R alpha
  • IL-6R alpha
  • IL-6R alpha
  • Inhibin
  • Insulin
  • ITIH4
  • Kallikrein 3 / PSA
  • Kallikrein 5
  • Kallikrein 6 / Neurosin
  • Lactoferrin
  • LBP
  • Leptin / OB
  • Leptin / OB
  • Leptin R
  • LIF
  • LIGHT / TNFSF14
  • Lipocalin-2 / NGAL
  • Lipocalin-2 / NGAL
  • LRG1
  • Lumican
  • Lymphotoxin-alpha / TNF-beta
  • M-CSF
  • M-CSF R / CD115
  • MAdCAM-1
  • MBL
  • MCAM / CD146
  • Mesothelin
  • MFG-E8
  • MIA
  • MICA
  • MICB
  • Midkine
  • MIF
  • MIF
  • MMP-1
  • MMP-2
  • MMP-3
  • MMP-7
  • MMP-8
  • MMP-9
  • MMP-10
  • MMP-12
  • MMP-13
  • MSP / MST1
  • Myeloperoxidase / MPO
  • Myoglobin
  • alpha-N-acetylglucosaminidase / NAGLU
  • NCAM-1 / CD56
  • Nectin-4
  • Nephrin
  • Neuregulin-1 beta 1 / NRG1 beta 1
  • Neuropilin-1
  • beta-NGF
  • NT-3
  • NT-4
  • Oncostatin M / OSM
  • Oncostatin M / OSM
  • Osteoactivin / GPNMB
  • Osteopontin / OPN
  • Osteopontin / OPN
  • Osteoprotegerin / TNFRSF11B
  • Park7 / DJ-1
  • PBEF / Visfatin
  • PD-L1 / B7-H1
  • PDGF-AA
  • PDGF-AB
  • PDGF-AB
  • PDGF-BB
  • PDGF-BB
  • PDGF-CC
  • PDGF-DD
  • Pentraxin 2 / SAP
  • Pentraxin 3 / TSG-14
  • Periostin / OSF-2
  • PLA2G7 / PAF-AH / Lp-PLA2
  • PlGF
  • Procalcitonin
  • Progranulin / PGRN
  • Prolactin
  • Properdin
  • Proprotein Convertase 9 / PCSK9
  • Protein S / PROS1
  • Proteinase 3 / Myeloblastin / PRTN3
  • RAGE / AGER
  • RBP4 / Retinol-Binding Protein 4
  • Reg3A
  • Relaxin-2
  • Renin
  • Resistin
  • ROBO4
  • S100A8
  • S100A9
  • S100B
  • SCF / c-kit Ligand
  • SCGF / CLEC11a
  • E-Selectin / CD62E
  • E-Selectin / CD62E
  • L-Selectin / CD62L
  • P-Selectin / CD62P
  • Serpin A4 / Kallistatin
  • Serpin A7 / TBG
  • Serpin A10 / ZPI
  • Serpin A12
  • Serpin B3 / SCCA1
  • Serpin C1 / Antithrombin-III
  • Serpin E1 / PAI-1
  • Serpin F1 / PEDF
  • SHBG
  • SLPI
  • SOST / Sclerostin
  • SP-D
  • SPARC
  • ST2 / IL-33R
  • Stanniocalcin 1 / STC-1
  • Syndecan-1 / CD138
  • Syndecan-4
  • alpha-Synuclein
  • TACI / TNFRSF13B
  • Tau
  • Tenascin C
  • TFF3
  • TFPI
  • TGF-alpha
  • Thrombomodulin / BDCA-3
  • Thrombopoietin / Tpo
  • Thrombospondin-2
  • Thymidine Kinase 1
  • Tie-2
  • TIM-1 / KIM-1 / HAVCR
  • TIMP-1
  • TNF-alpha
  • TNF-alpha
  • TNF RI / TNFRSF1A
  • TNF RII / TNFRSF1B
  • TRACP / PAP / ACP5
  • TRAIL / TNFSF10
  • TRAIL / TNFSF10
  • TRAIL R2 / TNFRSF10B
  • TRAIL R3 / TNFRSF10C
  • TRANCE / TNFSF11 / RANK L
  • TfR (Transferrin R)
  • TREM-1
  • TSLP
  • u-Plasminogen Activator (uPA) / Urokinase
  • UCH-L1 / PGP9.5
  • ULBP-1
  • ULBP-2 / 5 / 6
  • ULBP-3
  • ULBP-4 / RAET1E
  • uPAR
  • uPAR
  • Uromodulin
  • VAP-1 / AOC3
  • VCAM-1 / CD106
  • VEGF
  • VEGF
  • VEGF-C
  • VEGF-C
  • VEGFR1 / Flt-1
  • VEGFR2 / KDR / Flk-1
  • VEGFR3 / Flt-4
  • Vitamin D BP
  • XCL1 / Lymphotactin

Available Primate Analytes
  • BDNF
  • CCL2 / JE / MCP-1
  • CCL5 / RANTES
  • CCL11 / Eotaxin
  • CCL20 / MIP-3 alpha
  • CD40 Ligand / TNFSF5
  • CXCL2 / GRO beta / MIP-2 / CINC-3
  • CXCL10 / IP-10 / CRG-2
  • CXCL11 / I-TAC
  • CXCL13 / BLC / BCA-1
  • FGF basic / FGF2 / bFGF
  • G-CSF
  • GM-CSF
  • Granzyme B
  • IFN-alpha
  • IFN-beta
  • IFN-gamma
  • IL-1 beta / IL-1F2
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8 / CXCL8
  • IL-10
  • IL-12 p70
  • IL-13
  • IL-15
  • IL-17 / IL-17A
  • IL-21
  • PD-L1 / B7-H1
  • PDGF-AA
  • PDGF-BB
  • TGF-alpha
  • TNF-alpha
  • VEGF

Available Mouse Analytes
  • Adiponectin / Acrp30
  • Angiopoietin-2
  • BAFF / BLyS / TNFSF13B
  • C-Reactive Protein / CRP
  • C1qR1 / CD93
  • CCL2 / JE / MCP-1
  • CCL3 / MIP-1 alpha
  • CCL4 / MIP-1 beta
  • CCL5 / RANTES
  • CCL7 / MCP-3 / MARC
  • CCL8 / MCP-2
  • CCL11 / Eotaxin
  • CCL12 / MCP-5
  • CCL19 / MIP-3 beta
  • CCL20 / MIP-3 alpha
  • CCL21 / 6Ckine
  • CCL22 / MDC
  • Chitinase 3-like 1
  • Complement Factor D / Adipsin
  • CXCL1 / GRO alpha / KC / CINC-1
  • CXCL2 / GRO beta / MIP-2 / CINC-3
  • CXCL10 / IP-10 / CRG-2
  • CXCL12 / SDF-1 alpha
  • CXCL13 / BLC / BCA-1
  • CXCL16
  • Cystatin C
  • Dkk-1
  • DPPIV / CD26
  • EGF
  • EGFR
  • EMMPRIN / CD147
  • Endoglin / CD105
  • FABP4 / A-FABP
  • Fas Ligand / TNFSF6
  • FGF basic / FGF2 / bFGF
  • FGF-21
  • G-CSF
  • GDF-15
  • GM-CSF
  • Granzyme B
  • Haptoglobin
  • HGF
  • ICAM-1 / CD54
  • IFN-gamma
  • IGF-I / IGF-1
  • IGFBP-1
  • IGFBP-3
  • IL-1 alpha / IL-1F1
  • IL-1 beta / IL-1F2
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-10
  • IL-12 p70
  • IL-13
  • IL-16
  • IL-17 / IL-17A
  • IL-17E / IL-25
  • IL-27
  • IL-33
  • IL-6R alpha
  • LDLR
  • Leptin / OB
  • LIX
  • M-CSF
  • MMP-2
  • MMP-3
  • MMP-8
  • MMP-9
  • MMP-12
  • Nephrin
  • beta-NGF
  • Oncostatin M / OSM
  • Osteopontin / OPN
  • Osteoprotegerin / TNFRSF11B
  • Pancreatic Polypeptide / PP
  • PDGF-AA
  • PDGF-AB
  • PDGF-BB
  • Periostin / OSF-2
  • PlGF-2
  • Podocalyxin
  • Prolactin
  • Proprotein Convertase 9 / PCSK9
  • RAGE / AGER
  • Renin
  • Resistin
  • S100A8
  • S100A9
  • P-Selectin / CD62P
  • Serpin E1 / PAI-1
  • SP-D
  • Syndecan-1 / CD138
  • Thrombospondin-4
  • TIM-1 / KIM-1 / HAVCR
  • TIMP-1
  • TIMP-4
  • TNF-alpha
  • TNF RI / TNFRSF1A
  • TNF RII / TNFRSF1B
  • TRANCE / TNFSF11 / RANK L
  • TWEAK / TNFSF12
  • uPAR
  • VEGF
  • VEGFR2 / KDR / Flk-1

Available Rat Analytes
  • VEGFR2 / KDR / Flk-1
  • CXCL2 / GRO beta / MIP-2 / CINC-3
  • CXCL3 / GRO gamma / CINC-2 / DCIP-1
  • GM-CSF
  • ICAM-1 / CD54
  • IFN-gamma
  • IL-1 alpha / IL-1F1
  • IL-1 beta / IL-1F2
  • IL-2
  • IL-4
  • IL-6
  • IL-10
  • IL-13
  • IL-18 / IL-1F4
  • L-Selectin / CD62L
  • TIMP-1
  • TNF-alpha
  • VEGF

Luminex Assay Principle
Multiplex Luminex Assay Principle Step 1: The sample is added to color-coded beads, pre-coated with analyte-specific capture antibodies.

The sample is added to a mixture of color-coded beads, pre-coated with analyte-specific capture antibodies. The antibodies bind to the analytes of interest.
Biotinylated detection antibodies are added and form an antibody-antigen sandwich.

Biotinylated detection antibodies specific to the analytes of interest are added and form an antibody-antigen sandwich. Phycoerythrin (PE)-conjugated streptavidin is added. It binds to the biotinylated detection antibodies.

Beads are read on a dual-laser flow-based detection instrument.
Beads are read on a dual-laser flow-based detection instrument, such as the Luminex 200 or FlexMap® analyzer. One laser classifies the bead and determines the analyte that is being detected. The second laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound.
In addition to flow-based analyzers, magnetic beads can be read using the Luminex MAGPIX® Analyzer. A magnet in the MAGPIX analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct light-emitting diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and, the second LED determines the magnitude of the PE-derived signal. Each well is imaged with a CCD camera.
Customer Testimonials
Anh-Dung Le, CEO DermaTec LLC "The guys at BioAssay Systems know their science! I had to develop a bioassay for our company and Robert was very quick to respond to keep us up to date. They are knowledgeable and very resourceful. They directed us to one of the bulk suppliers that could give us a really good deal on our active ingredient. They keep very detailed records for our research needs. I would recommend working with BioAssay Systems on your assay development projects!"

Edward T. Wei, Chief Scientific Officer, Orinda Pharma, Inc.
"It is a pleasure working together with BioAssay Systems. I brought samples to their Hayward location and received the results in less than 24 hr. The discussion with Robert Z was courteous, open, and with a depth of knowledge. Robert has a PhD from Stanford. Also, Frank Huang, the CEO, had sufficient scientific curiosity to try out our cleanser and provide feedback! This is beyond the call of customer relations. BioAssay Systems is a company with a lot of expertise and a friendly, responsive approach to problem solving. I will enthusiastically continue to work with them to carry out our research."