QuantiFluo™ Protein Assay Kit
- For quantitative determination of total protein.
- Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 μg/mL BSA.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
- 200 tests
- 50 ng/mL
- 6 months
- The protein or peptide is known as the "building blocks of life" and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable. BioAssay Systems QuantiFluo™ protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (λex/em = 360/450nm) is proportional to the protein concentration in the sample.