QuantiChrom™ Phytase Assay Kit

Protocol SDS

Application

• A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples.

Key Features

• Simple and convenient: No complex detection reagents to mix.
• High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay.
• Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes.

Method

• OD620nm

Samples

• Enzyme extracts, agriculture and biological samples

Species

• All (e.g. Wheat, Barley)

Procedure

• 60 min

Size

• 100 tests

Detection Limit

• 0.01 U/L

Shelf Life

• 12 months

More Details

• PHYTASE catalyzes the hydrolysis of phosphoester bonds on myoinositol-(1,2,3,4,5,6)-hexakisphosphate (Phytic acid or IP6) thereby releasing inositol and phosphate. Phytic acid is a major storage reservoir for phosphate in plants. Phytase is abundant in grains such as wheat and barley, and the hydrolysis of phytic acid by single-stomached animals is a crucial aspect of animal nutrition. In addition, the lack of phytase in single-stomached animals can lead to excessive phosphorus leaching into the environment due to undigested phytic acid.
  
  Simple, direct and automation-ready procedures for measuring phytase activity are very desirable. BioAssay Systems' QuantiChromTM Phytase Assay is based on our Malachite Green Phosphate Assay (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from phytic acid.