EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit

EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit
Catalog No: ESHP2-100
Price: $459    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment:Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C
SHP2(Y542) Phosphorylation ELISA Kit
Protocol SDS

Application

  • Determination of SHP2(Y542) phosphorylation status in whole cells.
  • Evaluation of pathway modulation by activators, inhibitors, siRNA etc.

Key Features

  • New and improved. Total assay time reduced from the standard 9 hours to 5 hours.
  • Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary.
  • Accurate. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations.

Method

  • Cell-Based ELISA (FL530/585 nm, 360/450 nm)

Samples

  • Cells

Species

  • human

Size

  • 100 tests

Shelf Life

  • 6 months

More Details

  • PROTEIN PHOSPHORYLATION plays key roles in regulating protein activity within cells. A large number of protein kinases and phosphatases are known to be involved in a variety of signal transduction pathways. A rapid assay capable of monitoring such signal transduction pathways, target protein phosphorylation status and drug effects is highly desirable in life science research and drug discovery.

    Src Homology Region 2 Domain-containing Phosphatase-2 (SHP2, or SHPTP2, PTPN11, PTP2C) is a widely expressed protein-tyrosine phosphatase that plays regulatory roles in mitogenic activation, metabolic control, transcription regulation, and cell migration. BioAssay Systems' SHP2 (Y542) Phosphorylation ELISA Kit provides a rapid and sensitive assay for measuring the phosphorylated protein pSHP2(Y542) in human cells and normalizes the level of phosphorylation to the total protein content in the same well. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study the underlying signal pathway and effects of inhibitors, siRNA or activators on the pathway. In this assay, cells grown in a 96-well plate are fixed and permeabilized. The phosphorylation of SHP2(Y542) is measured using a specific primary antibody followed by a HRP-conjugated secondary antibody with a fluorogenic substrate, and a fluorescent reagent that measures the total protein in the same well.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.
Buy Now!!!
EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit
Catalog No: ESHP2-100
Price: $459     Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment:Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C
SHP2(Y542) Phosphorylation ELISA Kit

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