EnzyChrom™ Creatinine Assay Kit
- For quantitative determination of creatinine and evaluation of drug effects on its metabolism.
- Fast and sensitive. Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction. It is 3- and 53-fold more sensitive than the traditional Jaffe method (e.g. DICT-500), especially useful for small samples or where high sensitivity is required.
- Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day.
- OD570nm; FL530/585nm
- Urine, serum, plasma, and other biological preparations
- 60 min
- 100 tests
- Colorimetric assay: 4.8 µM or 0.054 mg/dL; Fluorimetric assay: 0.25 µM or 0.0028 mg/dL
- 6 months
- CREATININE is synthesized in the body from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and hence the urine levels are diminished. Creatinine testing is most widely used to assess kidney function.
BioAssay Systems' enzyme-based creatinine assay uses a reaction sequence that excludes endogenous ammonia to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the creatinine concentration in the sample.
What samples have you tested?This kit has been tested and validated in urine, plasma, and serum samples.
Can I store unused reagents for future use?Yes, unused reagents can be stored according to the assay protocol.
How do you use this kit on plasma or serum samples?Serum and Plasma samples should be deproteinated by centrifugation for 15 min at 14000 rpm at room temperature through a 10 kDa spin filter (e.g. Corning Cat# 431483). The filtrate can be assayed directly.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.