Protocol SDS

Application

• For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.

Key Features

• Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 100 U/L for 20 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species

• All

Size

• 100 tests

Detection Limit

• 0.5 U/L

Shelf Life

• 6 months

More Details

• ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe₄S₄] ²⁺ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia and diabetes. BioAssay Systems' aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.