EnzyChrom™ Aconitase Assay Kit
Protocol
SDS
Application
- For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 100 U/L for 20 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.
Method
- OD565nm
Samples
- Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species
- All
Size
- 100 tests
Detection Limit
- 0.5 U/L
Shelf Life
- 6 months
More Details
- ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia and diabetes. BioAssay Systems' aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Stein, C. S., Jadiya, P., Zhang, X., McLendon, J. M., Abouassaly, G. M., Witmer, N. H. & Boudreau, R. L. (2018). Mitoregulin: A lncRNA-encoded microprotein that supports mitochondrial supercomplexes and respiratory efficiency. Cell reports, 23(13), 3710-3720. Assay: Aconitase in human tissue.
To find more recent publications, please click here.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
